Fuel tank explosions have been a constant serious threat with aviation safety. Based on the analysis and study of the requirements of regulation and advisory circular which are about the airworthiness limitation for transport category airplane fuel system′s ignition source prevention, this paper has given the flow chart on how to establish the airworthiness limitation. The paper also analyzes the airworthiness limitation for an typical transport airplane fuel system′s ignition source prevention. The research can provide guidance for establishing the airworthiness limitation for transport category airplane fuel system′s ignition source prevention.