The development and verification of flight operation procedure is a complex and long-term task, which may involve various factors and considerations. Based on the practice of the procedure development of an airplane and the research on related references, this paper systematically presents a basic methodology of flight operation procedure development and evaluation for civil airplane. The related principles, constitution requirements, key elements and formats were introduced in details. The methodology is established for the procedure design specifically,which is an integration and abstraction of knowledge and practices in the area. It will provide a useful and effective guidance for the flight procedure development.